Raimondo D'Ambrosio, PhD

Portrait of Raimondo D'Ambrosio, Ph.D.
Job Title
Professor of Neurological Surgery





Research Interests

Dr. D'Ambrosio's research interests are in posttraumatic epilepsy. His research focuses on glial-neuronal-vascular interactions in models of head injury and epilepsy. He has NIH, DOD and private foundation research grant support.


Doctorate of Philosophy

University of Milan

Post-Doctoral Studies

University of Washington

Contact Information




  • Curia G, Eastman CL, Miller JW, D'Ambrosio R (2015) Modeling posttraumatic epilepsy for therapy development. In: Gerald A. Grant and Daniel Laskowitz, Eds. Translational Research in Traumatic Brain Injury, Frontiers in Neuroscience, Taylor & Francis Book / CRC Press, Boca Raton, Fl.  In press.
  • D’Ambrosio R Posttraumatic Epilepsy Caused by Fluid Percussion Injury. In: Philip A. Schwartzkroin, editor Encyclopedia of Basic Epilepsy Research, Vol 3. Oxford: Academic Press; 2009. pp. 1224-1229.
  • D’Ambrosio R. (2003) Basic science of posttraumatic epilepsy. In: Youman’s Neurological Surgery. 5th edition, H.R. Winn ed., pp2449-2460. Elsevier.
  • D’Ambrosio R. (2002) The perforated patch technique. In: Neuromethods: Patch Clamp Applications and Protocols. Boulton AA, Baker GB, and Walz W eds. The Humana Press.
  • Gasparini S, Maccaferri G, D’Ambrosio R., and DiFrancesco D (1996) The hyperpolarization-activated current (Ih/Iq) in rat hippocampal neurons. In: Neurobiology: Ionic Channels, Neurons and the brain, V. Torre and F. Conti eds., pp 63-74, Plenum Publishing Corporation.


  • Kadam SD, D'Ambrosio R, Duveau V, Roucard C, Garcia-Cairasco N, Ikeda A, de Curtis M, Galanopoulou AS, Kelly KM. Methodological standards and interpretation of video-EEG in adult control rodents.A TASK1-WG1 report of the AES/ILAE Translational Task Force of the ILAE. Epilepsia, in press.
  • Tai TY, Warner LN, Jones TD, Jung S, Concepcion FA, Skyrud DW, Fender J, Liu Y, Williams AD, Neumaier JF, D'Ambrosio R, Poolos NP (2017) Antiepileptic action of c-Jun N-terminal kinase (JNK) inhibition in an animal model of temporal lobe epilepsy. Neuroscience. 349:35-47. PMID: 28237815.
  • Smyth MD, Han RH, Yarbrough CK, Patterson EE, Yang XF, Miller JW, Rothman SM, D'Ambrosio R. (2015) Temperatures achieved in human and canine neocortex during intraoperative passive or active focal cooling. Ther Hypothermia Temp Manag. 5(2):95-103. doi: 10.1089/ther.2014.0025. Epub 2015 Apr 22.
  • Eastman CL, Fender JS, Temkin NR, D’Ambrosio R (2015) Optimized methods for epilepsy therapy development using an etiologically realistic model of focal epilepsy in the rat. Experimental Neurology. 2015, 264:150-62. PMID: 25523813.
  • Gupta PK, Ding K, Sayed N, Agostini MA, Van Ness PC, Madden C, Mickey B, D'Ambrosio R, Diaz-Arrastia R (2014) Subtypes of post-traumatic epilepsy: clinical, electrophysiologic, and imaging features. Journal of Neurotrauma, 2014 Apr 2. [Epub ahead of print] PMID: 24693960.
  • D’Ambrosio R, Eastman CL, Fattore C, Perucca E. Novel frontiers in epilepsy treatments: preventing epileptogenesis by targeting inflammation. Expert Review of Neurotherapeutics, 2013 Jun;13(6):615-25. doi: 10.1586/ern.13.54. PMID: 23738999.
  • D’Ambrosio R, Eastman CL, Darvas F, Fender JS, Verley DR, Farin, FM, Wilkerson HW, Temkin NR, Miller JW, Ojemann J, Rothman SM, Smyth MD. Mild passive focal cooling prevents epileptic seizures after head injury in rats. Annals of Neurology, 2013 Feb; 73(2):199-209. doi: 10.1002/ana.23764. Epub 2012 Dec 7. F1000Prime article. PMID: 23225633.
  • Hakimian S, Miller JW, Ojemann J, Hebb AO, D’Ambrosio R, Ojemann G, Kershenovich A. Long term outcome of extratemporal resection of posttraumatic epilepsy. Neurosurg Focus. 2012 Mar;32(3):E10. doi: 10.3171/2012.1.FOCUS11329. PMID: 22380851.
  • Eastman CL, Verley DR, Fender JS, Stewart TH, Nov E, Curia G, and D’Ambrosio R. Antiepileptic and Antiepileptogenic Performance of Carisbamate after Head Injury in The Rat: Blind and Randomized Studies. Journal of Pharmacology and Experimental Therapeutics. 2011, 336(3):779-90. doi: 10.1124/jpet.110.175133. Epub 2010 Dec 1.
  • Curia G, Levitt M, Fender JS, Miller JW, Ojemann J, D’Ambrosio R. Impact of injury location and severity on posttraumatic epilepsy in the rat: role of frontal neocortex. Cerebral Cortex, 2010, Nov 26. [Epub ahead of print]. Cereb Cortex. 2011 Jul;21(7):1574-92. PMID: 21112931.
  • Stewart TH, Eastman CL, Groblewski PA, Fender JS, Verley DR, Cook DG, D’Ambrosio R. Chronic dysfunction of astrocytic inwardly rectifying K+ channels specific to the neocortical epileptic focus after fluid percussion injury in the rat. Journal of Neurophysiology, 2010, 104(6):3345-60. PMID: 20861444.
  • Eastman CL, Verley DR, Fender JS, Temkin NR and D’Ambrosio R. ECoG studies of valproate, carbamazepine and halothane in frontal lobe epilepsy induced by head injury in the rat. Experimental Neurology. 2010, 224(2):369-88. PMID: 20420832; PMCID 2906631.
  • D’Ambrosio R, and Miller JW. What is an Epileptic Seizure? Unifying Definitions in Clinical Practice and Animal Research to Develop Novel Treatments. Epilepsy Currents, 2010, 10(3):61-66. PMID: 20502593. Answer in Epilepsy Currents, 2010, 10(4):90. PMID: 20697503. PMCID 2873641.
  • D’Ambrosio R, Hakimian, S, Stewart T, Verley DR, Fender JS, Eastman CL, Sheerin AH, Gupta P, Diaz-Arrastia R, Ojemann J, Miller JW. Functional Definition of Seizures Provides New Insights into Posttraumatic Epileptogenesis. Brain, 2009, 132(Pt 10):2805-21. PMID: 19755519; PMCID 2759339. Cover article.
  • Jung S, Jones TD, Lugo JN Jr, Sheerin AH, Miller JW, D'Ambrosio R, Anderson AE, Poolos NP.Progressive dendritic HCN channelopathy during epileptogenesis in the rat pilocarpine model of epilepsy. J Neurosci. 2007, 27(47):13012-21. PMID: 18032674.
  • Miller JW and D'Ambrosio R (2007) When Basic Research Doesn’t Translate to the Bedside—Lessons from the Magnesium Brain Trauma Study. Epilepsy Currents, 7(5):133-5.
  • D'Ambrosio R (2006) Does glutamate released by astrocytes cause focal epilepsy? Epilepsy Currents, 6(5):12-15.
  • D’Ambrosio R, Fender JS, Fairbanks JP, Simon E, Born DE, Doyle D, Miller JW. Progression from frontal-parietal to mesial-temporal epilepsy after fluid percussion injury in the rat. Brain, 2005, 128(Pt 1):174-88. PMID: 15563512; PMCID 2696356.
  • D'Ambrosio R. (2005) A possible causative role for blood brain barrier failure and reactive astrocytosys in acquired epilepsy. Epilepsy Currents, 5(6):244-246.
  • D’Ambrosio R and Perucca E. Epilepsy after head injury. Current Opinion in Neurology, 2004, 17(6):731-735. PMID:15542983; PMCID 2672045.
  • Progression of posttraumatic epilepsy following fluid percussion injury. D’Ambrosio R. In: Loscher W, Schmidt D (2004) New horizons in the development of antiepileptic drugs. Epilepsy Research, Vol 60(2-3):145-148. NIHMSID # 105390.
  • D’Ambrosio R (2004) The role of glial ion channels in seizures and epileptogenesis. Pharmacology & Therapeutics, 103(2):95-108.
  • D’Ambrosio R, Fairbanks JP, Fender JS, Born DE, Doyle D and Miller JW. Posttraumatic epilepsy following fluid percussion injury in the rat. Brain 2004, 127(Pt 2):304-14. PMID: 14607786.
  • Meno JR, Higashi H, Cambray AJ, Zhou J, D’Ambrosio R and Winn HR. Hippocampal injury and neurobehavioral deficits are improved by PD 81,723 following hyperglycemic cerebral ischemia. Experimental Neurology, (2003):183(1):188-96.
  • Iliff JJ, D’Ambrosio R, Ngai AC, and Winn HR. Adenosine receptors mediate glutamate-evoked arteriolar dilation in the rat cerebral cortex. American Journal of Physiology, (2003) 284(5):H1631-1637.
  • D’Ambrosio R., Gordon D.S., and Winn H.R. (2002) Differential roles of KIR channel and Na+/K+-pump in the regulation of extracellular K+ accumulation in rat hippocampus. Journal of Neurophysiology, Vol 87: 87-102. PMID: 11784732.
  • D’Ambrosio R., Maris D.O., Grady S.M., Winn H.R., and Janigro D. (1999) Impaired K+ homeostasis and altered electrophysiological properties of post-traumatic hippocampal glia. Journal of Neuroscience, 19(18): 8152-62. PMID: 9614220.
  • Frequency-dependent changes in cerebral blood flow and evoked potentials during somatosensory stimulation in the rat. Ngai A.C., Jolley M.A., D’Ambrosio R., Meno J.R. and Winn H.R. Brain Research (1999), 837:221-228.
  • Hochman D.W., D’Ambrosio R., Janigro D., and Schwartzkroin P.A. (1999) Extracellular chloride and the maintenance of spontaneous epileptiform activity in rat hippocampal slices. Journal of Neurophysiology, 81:49-59. PMID: 9914266.
  • D’Ambrosio R., Wenzel J., Schwartzkroin P.A., McKhann G. II and Janigro D. (1998) Functional specialization and topographic segregation of three types of hippocampal astrocytes. Journal of Neuroscience, 18(12):4425-38. PMID: 9614220.
  • D’Ambrosio R., Maris D.O., Grady M.S., Winn H.R. and Janigro D. (1998) Selective loss of Long-Term Potentiation, but not Depression, following fluid percussion injury. Brain Research, Vol. 786 (1-2) pp. 64-79. PMID: 9554957.
  • Heterogeneity of astrocyte resting membrane potentials and intracellular coupling revealed by whole cell and gramicidin-perforated patch recordings from cultured neocortical and hippocampal slice astrocytes. McKhann G. II, D’Ambrosio R. and Janigro D. Journal of Neuroscience (1997), 17(18): 6850-63.
  • Reduction of K+ uptake in glia prevents Long-Term Depression maintenance and causes epileptiform activity. Janigro D., Gasparini S., D’Ambrosio R., McKhann G. II and DiFrancesco D. Journal of Neuroscience (1997), 17(8): 2813-24.